Full CDMO service

Orthros Medical is specialized in production of VHH in yeast. Our experience allows us to optimize VHH production to deliver a VHH with the best manufacturing and functional characteristics. 

The Orthros Medical lab in Raalte provides full service development and manufacturing of VHH. Starting from an idea or with an actual clone, the Orthros Medical team is ready to develop and produce your VHH. 

The way we work

VHH discovery

Orthros Medical focusses on the production of VHH antibodies through fed-batch fermentation. We modify the genomic DNA of the yeast train, Saccharomyces cerevisiae to express the VHH protein and excrete this in its environment. The excreted VHH is easily obtained, purified and afterwards is ready for application in your research or further development. Our laboratory  quality system ensures a reliable and qualitative product. We offer a range of services within VHH production process. 

VHH characterization

The VHH are initially selected by ELISA with its corresponding antigen. This step in the process is carried out by our partner, QVQ Holding B.V. The best binders are selected after coating the well plate with the desired antigen. 

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The DNA from the best binders are obtained and optimised for production in yeast. During the optimisation process the VHH is checked throughout VHH optimisation for specific host organism is possible. By host optimisation, lama VHH is adapted to the host organism e.g. human, dogs or horses. 

Before producing the VHH, bioinformatics is conducted to obeserve the possible 3D structure of the VHH. Virtual docking of the VHH to the desired antigen is also possible to predict the VHH binding spot to the antigen.

Quality Assurance

The DNA of the VHH is control in every step of the process to unsure that no nonsense mutation has taken place during the process. 

VHH manufacturing

We produce our VHH by genetically modifying the genomic DNA of the yeast strain, Saccharomyces cerevisiae to express and secrete this protein. Saccharomyces cerevisiae is a well characterized organism for the production of heterologous protein. As a result, the system is enhanced by engineering of the host strain to overexpress VHH. Yeast has the ability to perform post translational modifications